■ 技術情報
■ 製品説明書
- C-Kit Ground Pro XRD: X線回折実験向けのタンパク質結晶作成研究用キットです。＞＞C-Kit Ground Pro XRDの取扱説明書はこちら
- C-Kit Ground Pro ND: 中性子線回折実験向けのタンパク質結晶作成研究用キットです。＞＞C-Kit Ground Pro NDの取扱説明書はこちら
■ 実験操作動画
■ C-Kit Education
- C-Kit Educationの実験操作の説明動画です
■ C-Tube LCDM
- C-Tube LCDMの充填方法の説明動画です
■ バッチ法
- ガラスキャピラリーを利用したバッチ法の充填方法の説明動画です
■ 外部発表リスト
■ 宇宙実験：微小重力効果
- 宇宙環境を利用したタンパク質結晶化実験の技術的背景 Background of protein crystallization technologies in space. 高橋幸子, 田仲広明. Int. J. Microgravity Sci. Appl. (2017). 34(1), 340103.(Japanese)
- 宇宙ならびに地上で生成したタンパク質結晶のX線回折能の不均一性 Non-uniform diffractive quality of the crystals grown in microgravity and in terrestrial gravity. 高橋幸子, 中川敦史, 古林直樹, 伊中浩治, 太田和敬, 田仲広明. 日本結晶成長学会誌 (2016). 43(2), 47–53.(Japanese)
- Numerical model of protein crystal growth in a diffusive field such as the microgravity environment. H. Tanaka, S. Sasaki, S. Takahashi, K. Inaka, Y. Wada, M. Yamada, K. Ohta, H. Miyoshi, T. Kobayashi and S. Kamigaichi, J. Synchrotron Rad. (2013). 20, 1003–1009.
- Numerical analysis of the diffusive field around a growing protein crystal in microgravity. K. Inaka, H. Tanaka, S. Takahashi, S. Sano, M. Sato, M. Shirakawa and Y. Yoshimura. Defect and Diffusion Forum Vols. 323-325 (2012). pp565-569.
- Controlling the Diffusive Field to Grow a Higher Quality Protein Crystal in Microgravity. H. Tanaka, K. Inaka, N. Furubayashi, M. Yamanaka, S. Takahashi, S. Sano, M. Sato, M. Shirakawa and Y. Yoshimura, Defect and Diffusion Forum Vols. 323-325 (2012). pp549-554.
- 宇宙実験での蛋白質結晶の高品質化事前予測技術. 田仲広明、伊中浩治、古林直樹、佐藤勝、高橋幸子、山中麻里、広田恵理華、佐野智、小林智之、田中哲夫日本マイクログラビティ応用学会誌 (2008). 25(2),101-106 (Japanese).
- 宇宙環境を利用した高品質なタンパク質結晶の生成. 高橋幸子、田仲広明, 日本結晶学会誌(2004). 46, 323-331 (Japanese).
- Numerical Analysis of the Depletion Zone Formation Around a Growing Protein Crystal. Hiroaki Tanaka, Koji Inaka, Shigeru Sugiyama, Sachiko Takahashi, Satoshi Sano, Masaru Sato, Susumu Yoshitomi. Transport Phenomena in Microgravity (2004), 1027(1), 10-19.
■ 宇宙実験技術:容器
- JCB-SGT Crystallization Devices Applicable to PCG Experiments and their Crystallization Conditions. Sachiko TAKAHASHI, Misako KOGA, Bin YAN, Naoki FURUBAYASHI, Masayuki KAMO, Koji INAKA and Hiroaki TANAKA. Int. J. Microgravity Sci. Appl. (2019). 36(1), 360107.
■ 宇宙実験技術:実験プロジェクト
- 構造解析向けタンパク質結晶化宇宙実験に必要な実験技術 Essential Techniques for growing high-quality protein crystals for structural analysis in microgravity. 伊中浩治, 高橋幸子, 田仲広明. Int. J. Microgravity Sci. Appl. (2017). 34(1), 340104.(Japanese)
- JAXA protein crystallization in space: ongoing improvements for growing high-quality crystals. S. Takahashi, K. Ohta, N. Furubayashi, B. Yan, M. Koga, Y. Wada, M. Yamada, K. Inaka, H. Tanaka, H. Miyoshi, T. Kobayashi and S. Kamigaichi, J. Synchrotron Rad. (2013). 20, 968–97.
- 「きぼう」を利用した高品質タンパク質結晶生成実験の現状と合理的な宇宙実験へのアプローチ. 高橋幸子、厳斌、古林直樹、正木美佳、太田和敬、伊中浩治、田仲広明、小林智之、吉村善範. 日本マイクログラビティ応用学会誌 (2012).29(3),111-119.
- 国際宇宙ステーションを利用した高品質タンパク質結晶生成実験技術のスピンオフ. 田仲広明、高橋幸子、古林直樹、山中麻里、厳斌、広田恵理華、佐野智、佐藤勝、伊中浩治、小林智之、田中哲夫.日本マイクログラビティ応用学会誌 (2010). 27(2), 104-112.
- JAXA-GCF project - High-quality protein crystals grown under microgravity environment for better understanding of protein structure. Sato, M., Tanaka, H., Inaka, K. et al. Microgravity Sci. Technol 18, 184–189 (2006).
■ 宇宙実験技術:成果
- Effect of Impurity Concentration on the Precipitation of Atorvastatin Calcium Under Microgravity on the International Space Station. Makoto Otsuka, Yota Kuroyanagi, Tetsuo Sasaki, Masakazu Matsumoto, Yutaro Hirose, Hiroaki Tanaka and Naohiro Sato. Colloids and Surfaces A: Physicochemical and Engineering Aspects (2024) 134553.
- Microgravity environment grown crystal structure information based engineering of direct electron transfer type glucose dehydrogenase. Junko Okuda-Shimazaki, Hiromi Yoshida, Inyoung Lee, Katsuhiro Kojima, Nanoha Suzuki, Wakako Tsugawa, Mitsugu Yamada, Koji Inaka, Hiroaki Tanaka and Koji Sode. Communications Biology 5 (2022) 1334.
- Fragment-based discovery of the first nonpeptidyl inhibitor of an S46 family peptidase. Yasumitsu Sakamoto, Yoshiyuki Suzuki, Akihiro Nakamura, Yurie Watanabe, Mizuki Sekiya, Saori Roppongi, Chisato Kushibiki, Ippei Iizuka, Osamu Tani, Hitoshi Sakashita, Koji Inaka, Hiroaki Tanaka, Mitsugu Yamada, Kazunori Ohta, Nobuyuki Honma, Yosuke Shida, Wataru Ogasawara, Mayumi Nakanishi-Matsui, Takamasa Nonaka, Hiroaki Gouda & Nobutada Tanaka. Scientific Reports volume 9, Article number: 13587 (2019).
- Crystal Structure of Chitinase ChiW from Paenibacillus sp. str. FPU-7 Reveals a Novel Type of Bacterial Cell-Surface-Expressed Multi-Modular Enzyme Machinery. Itoh T, Hibi T, Suzuki F, Sugimoto I, Fujiwara A, et al. PLOS ONE (2016) 11(12): e0167310.
- “Newton’s cradle” proton relay with amide–imidic acid tautomerization in inverting cellulase visualized by neutron crystallography. Akihiko Nakamura, Takuya Ishida, Katsuhiro Kusaka, Taro Yamada, Shinya Fushinobu, Ichiro Tanaka, Satoshi Kaneko, Kazunori Ohta, Hiroaki Tanaka, Koji Inaka, Yoshiki Higuchi, Nobuo Niimura, Masahiro Samejima and Kiyohiko Igarashi. SCIENCE ADVANCES21 AUG 2015 : E1500263.
- Structural and mutational analyses of dipeptidyl peptidase 11 from Porphyromonas gingivalis reveal the molecular basis for strict substrate specificity. Yasumitsu Sakamoto, Yoshiyuki Suzuki, Ippei Iizuka, Chika Tateoka, Saori Roppongi, Mayu Fujimoto, Koji Inaka, Hiroaki Tanaka, Mitsugu Yamada, Kazunori Ohta, Hiroaki Gouda, Takamasa Nonaka, Wataru Ogasawara & Nobutada Tanaka. Scientific Reports volume 5, Article number: 11151 (2015).
- S46 Peptidases are the First Exopeptidases to be Members of Clan PA. Yasumitsu Sakamoto, Yoshiyuki Suzuki, Ippei Iizuka, Chika Tateoka, Saori Roppongi, Mayu Fujimoto, Koji Inaka, Hiroaki Tanaka, Mika Masaki, Kazunori Ohta, Hirofumi Okada, Takamasa Nonaka, Yasushi Morikawa, Kazuo T. Nakamura, Wataru Ogasawara & Nobutada Tanaka . Sci Rep 4, 4977 (2014).
- High-Quality Protein Crystal Growth of Mouse Lipocalin- Type Prostaglandin D Synthase in Microgravity. K. Inaka, S. Takahashi, K. Aritake, T. Tsurumura, N. Furubayashi, B. Yan, E. Hirota, S. Sano, M. Sato, T. Kobayashi, Y. Yoshimura, H. Tanaka and Y. Urade, Cryst Growth Des. (2011). June 1; 11(6): 2107-2111.
- Improvement in the quality of hematopoietic prostaglandin D synthase crystals in a microgravity environment. H. Tanaka, T. Tsurumura, K. Aritake, N. Furubayashi, S. Takahashi, M. Yamanaka, E. Hirota, S. Sano, M. Sato, T. Kobayashi, T. Tanaka, K. Inaka and Y. Urade. J. Synchrotron Rad. (2011). 18, 88-91.
- High-quality crystals of human haematopoietic prostaglandin D synthase with novel inhibitors. S. Takahashi, T. Tsurumura, K. Aritake, N. Furubayashi, M. Sato, M. Yamanaka, E. Hirota, S. Sano, T. Kobayashi, T. Tanaka, K. Inaka, H. Tanaka and Y. Urade. Acta Cryst. (2010). F66, 846-850.
- ISSを利用した高品質タンパク質結晶生成実験の応用利用. 高橋幸子、佐野智、佐藤勝、広田恵理華、古林直樹、厳斌、小林智之、伊中浩治、田仲広明、田中哲夫日本マイクログラビティ応用学会誌 (2010). 27(2), 98-103.
- 宇宙実験による高分解能タンパク質構造解析の応用に向けて. 高橋幸子、古林直樹、鶴村俊治、有竹浩介、佐藤勝、山中麻里、広田恵理華、佐野智、伊中浩治、田仲広明、小林智之、裏出良博、田中哲夫.日本マイクログラビティ応用学会誌 (2008).25(2),141-146.
- Crystallization of the archaeal transcription termination factor NusA: a significant decrease in twinning under microgravity conditions. H. Tanaka, T. Umehara, K. Inaka, S. Takahashi, R. Shibata, Y. Bessho, M. Sato, S. Sugiyama, E. Fusatomi, T. Terada, M. Shirouzu, S. Sano, M. Motohara, T. Kobayashi, T. Tanaka, A. Tanaka and S. Yokoyama. Acta Cryst. (2007). F63, 69-73.
■ 結晶化関連技術
- Through Diffusion Measurements of Molecules to a Numerical Model for Protein Crystallization in Viscous Polyethylene Glycol Solution. Hiroaki Tanaka, Rei Utata, Keiko Tsuganezawa, Sachiko Takahashi and Akiko Tanaka. Crystals (2022). 12, 881.
- Novel Device and Strategy for Growing Large, High-Quality Protein Crystals by Controlling Crystallization Conditions. Naoki Tanigawa, Sachiko Takahashi, Bin Yan, Masayuki Kamo, Naoki Furubayashi, Koji Kubota, Koji Inaka and Hiroaki Tanaka. Crystals (2021). 11, 1311.
- Semi-empirical model to estimate ideal conditions for the growth of large protein crystals. Hirohiko Nakamura, Sachiko Takahashi, Koji Inaka and Hiroaki Tanaka. Acta Cryst. D. 76 (2020) 1174-1183.
- Methods for Obtaining Better Diffractive Protein Crystals: From Sample Evaluation to Space Crystallization. Yoshinobu. Hashizume, Koji Inaka, Naoki Furubayashi, Masayuki Kamo, Sachiko Takahashi and Hiroaki Tanaka. Crystals (2020). 10, 78.
- A novel handling-free method of mounting single protein crystals for synchrotron structure analyses at room temperature. Yoshihisa Suzuki, Kumi Torii, Koji Inaka, Takahisa Fujiwara, Hiroaki Tanaka and Yoshitomo Arai. Review of Scientific Instruments 90, 054101 (2019).
- Optimization of salt concentration in PEG-based crystallization solutions. M. Yamanaka, K. Inaka, N. Furubayashi, M. Matsushima, S. Takahashi, H. Tanaka, S. Sano, M. Sato, T. Kobayashi and T. Tanaka. J. Synchrotron Rad. (2011). 18, 84-87.
- Diffusion coefficient of the protein in various crystallization solutions: The key to growing high-quality crystals in space. Hiroaki Tanaka, Sachiko Takahashi, Mari Yamanaka, Izumi Yoshizaki, Masaru Sato, Satoshi Sano, Moritoshi Motohara, Tomoyuki Kobayashi, Susumu Yoshitomi, Tetsuo Tanaka and Seijiro Fukuyama. Microgravity Sci. Technol 18, 91–94 (2006).
- A simplified counter diffusion method combined with a 1D simulation program for optimizing crystallization conditions. H. Tanaka, K. Inaka, S. Sugiyama, S. Takahashi, S. Sano, M. Sato and S. Yoshitomi. J. Synchrotron Rad. (2004). 11, 45-48.
■ SETM教育
- 日本の宇宙環境利用とSTEM教育. 田仲広明. レデックスメルマガ. 2024年1月19日
- 国際宇宙ステーションを使ったSTEM教育への動機づけ. 田仲広明. レデックスメルマガ. 2024年1月5日