Expression and purification | We express protein in E.coli or in insect cells and purify to crystallization quality in alliance with Wako Pure Chemical Industries, Ltd. |
Unique initial crystallization trial | For rational approach, we initially try the crystallization condition efficiently with smaller amount of protein solution, using our own database and unique optimization steps. |
High-quality protein crystallization | Especially if you have already obtained a crystal which can diffract X-ray about 2 Åresolution, we apply various techniques to obtain crystals of about 1 Å resolution. We have a lot of experiences to handle crystals which diffracted beyond 1 Å resolution,k through technical support of Japan Aerospace Exploration Agency (JAXA) PCG project. We notably have a lot of experiences to purify protein to crystallization-grade. |
Counter-diffusion method for crystallization | Not only popular vapour-diffusion method, but also counter-diffusion method is used for crystallization in our C-Platform. The counter-diffusion method is adopted as a high-reproducible standard crystallization method in JAXA PCG. C-Tube is a crystallization device of the counter-diffusion method. It will help obtaining crystals of protein-ligand complexes by soaking ligands into the crystals. |
X-ray data collection | After obtaining good crystals, we collect X-ray diffraction data using synchrotron radiation facility, and hand F data to customers after scaling. |
Structure refinement | We have a lot of experiences of structure determination and analysis of X-ray diffraction data which is sometimes beyond 0.8 A resolution. |
Features that other companies do not have | Even if the crystallization did not work out well, we will examine the reasons and feed them back to customers, which will benefit to our customers to go forward to the next step. We will apply for the space crystallization experiment if our customer requests. |
Protein that have never been crystallized | Protein expression We express crystallization-grade protein in alliance with Wako Pure Chemical Industries, Ltd. Purification and verification of protein grade We purify protein to crystallization grade. Initial crystallization trial For rational approach, we initially try the crystallization condition efficiently with smaller amount of protein solution, using our own database and unique optimization steps. |
Protein that have already been crystallized will be improved more! | Protein expression If necessary, we express crystallization-grade protein in alliance with Wako Pure Chemical Industries, Ltd. Purification and verification of protein grade We purify protein to crystallization grade. Optimization of crystallization condition We optimize crystallization condition for higher-quality protein crystal. Space crystallization If desired, crystallization in space is performed. X-ray diffraction data collection Opportunity for X-ray diffraction data collection is available all year around using synchrotron radiation facility in Japan or in other country. If necessary, we offer support service for data collection at SPring-8. Structural analysis We can provide data of three dimensional atomic coordinate, hydrogen bond analysis, and interaction analysis of the protein with ligands, etc., and construct structure data which can be shown in three-dimension. |
Crystallization of protein-ligand complex | Crystallization of the complex We have a lot of know-how in crystallizing protein-ligand complexes. X-ray diffraction data collection We can offer the crystallization of a protein with several ligands and X--ray diffraction data collection with reasonable price. |
Experimental techniques | |
Maruwa Foods and Biosciences Inc. | Wako Pure Chemical |
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